The article concerns the maintenance of ecological connectivity of regional importance, as illustrated with the example of the Wieliszew municipality in the vicinity of Warsaw. The study consisted in analysing the landscape structure of the municipality, determining the existing natural connections and assessing their spatial continuity and functioning. The developed ecological network was confronted with the planned functional and spatial structure according to the study of spatial development conditions and directions, in particular in terms of the spread of anthropogenic barriers. The planned dense built development and new road of regional importance lead to significant fragmentation of the landscape, and thus result in limitation and the decrease in efficiency of the existing ecological connections.